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Proteins & DNA/RNA

Fibril Charge Affects α-Synuclein Hydrogel Rheological Properties

Pogostin, Brett H.; Linse, Sara; Olsson, Ulf

(Hydro)gelsApplicationCharacterizationCustomer publicationsMaterialNanoparticles & colloidsProteins & DNA/RNASAXSShapeSizeTechniqueWAXS

Fibril Charge Affects α-Synuclein Hydrogel Rheological Properties

Pogostin, Brett H.; Linse, Sara; Olsson, Ulf

In this paper, we have investigated the interactions between ?-synuclein fibrils at different pH values and how this relates to hydrogel formation and gel properties. Using a combination of rheology, small-angle X-ray scattering, Raman spectroscopy, and cryo-transmission electron microscopy (cryo-TEM) experiments, we have been able to investigate the relationship between…
Probing the Nanostructure and Arrangement of Bacterial Magnetosomes by Small-Angle X-Ray Scattering

Rosenfeldt, Sabine; Riese, Cornelius N.; Mickoleit, Frank; Schüler, Dirk; Schenk, Anna S.

ApplicationCharacterizationCustomer publicationsInorganic materialMaterialParticlesProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Probing the Nanostructure and Arrangement of Bacterial Magnetosomes by Small-Angle X-Ray Scattering

Rosenfeldt, Sabine; Riese, Cornelius N.; Mickoleit, Frank; Schüler, Dirk; Schenk, Anna S.

Magnetosomes are membrane-enveloped single-domain ferromagnetic nanoparticles enabling the navigation of magnetotactic bacteria along magnetic field lines. Strict control over each step of biomineralization generates particles of high crystallinity, strong magnetization, and remarkable uniformity in size and shape, which is particularly interesting for many biomedical and biotechnological…
Tandem catalysis in multicomponent solvent-free biofluids

Atkins, Dylan Luke; Berrocal, José Augusto; Mason, Alexander Francesco; Voets, Ilja Karina

ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Tandem catalysis in multicomponent solvent-free biofluids

Atkins, Dylan Luke; Berrocal, José Augusto; Mason, Alexander Francesco; Voets, Ilja Karina

Enzymes are widely employed to reduce the environmental impact of chemical industries as biocatalysts improve productivity and offer high selectively under mild reaction conditions in a diverse range of chemical transformations. The poor stability of biomacromolecules under reaction conditions is often a critical bottleneck to their application. Protein engineering or…
Studying Excipient Modulated Physical Stability and Viscosity of Monoclonal Antibody Formulations Using Small-Angle Scattering

Xu, Amy Yuanyuan; Castellanos, Maria Monica; Mattison, Kevin; Krueger, Susan; Curtis, Joseph E.

ApplicationCharacterizationCustomer publicationsMaterialParticle interactionsProteins & DNA/RNASAXSStructural biologyTechnique

Studying Excipient Modulated Physical Stability and Viscosity of Monoclonal Antibody Formulations Using Small-Angle Scattering

Xu, Amy Yuanyuan; Castellanos, Maria Monica; Mattison, Kevin; Krueger, Susan; Curtis, Joseph E.

Excipients are substances that are added to therapeutic products to improve stability, bioavailability, and manufacturability. Undesirable protein-protein interactions (PPI) can lead to self-association and/or high solution viscosity in concentrated protein formulations that are typically greater than 50 mg/mL. Therefore, understanding the effects of excipients on nonspecific PPI is important for…
Reproducible Preparation of Proteopolymersomes via Sequential Polymer Film Hydration and Membrane Protein Reconstitution

Loo, Siew-Leng; Siti, Winna; Thiyagarajan, Monisha; Torres, Jaume; Wang, Rong; Hu, Xiao

(Natural) polymersApplicationCharacteristic dimensionCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSStructural biologyTechnique

Reproducible Preparation of Proteopolymersomes via Sequential Polymer Film Hydration and Membrane Protein Reconstitution

Loo, Siew-Leng; Siti, Winna; Thiyagarajan, Monisha; Torres, Jaume; Wang, Rong; Hu, Xiao

Film rehydration method is commonly used for membrane protein (MP) reconstitution into block copolymer (BCP), but the lack of control in the rehydration step formed a heterogeneous population of proteopolymersomes that interferes with the characterization and performance of devices incorporating them. To improve the self-assembly of polymersomes with simultaneous MP…
Effects of charge contrast and composition on microgel formation and interactions with bacteria-mimicking liposomes

Borro, Bruno C.; Toussaint, Marie S.; Bucciarelli, Saskia; Malmsten, Martin

(Hydro)gelsApplicationCharacterizationCustomer publicationsLipidsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Effects of charge contrast and composition on microgel formation and interactions with bacteria-mimicking liposomes

Borro, Bruno C.; Toussaint, Marie S.; Bucciarelli, Saskia; Malmsten, Martin

Microgels offer opportunities for improved delivery of antimicrobial peptides (AMP). To contribute to a foundation for rational design of such systems, we here study the effects of electrostatics on the generation of peptide-carrying microgels. For this, alginate microgels loaded with polymyxin B and cross-linked by Ca2+, were formed by electrostatic…
Aescin-Induced Conversion of Gel-Phase Lipid Membranes into Bicelle-like Lipid Nanoparticles

Geisler, Ramsia; Pedersen, Martin Cramer; Hannappel, Yvonne; Schweins, Ralf; Prévost, Sylvain; Dattani, Rajeev; Arleth, Lise; Hellweg, Thomas

ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologySurfactantsTechnique

Aescin-Induced Conversion of Gel-Phase Lipid Membranes into Bicelle-like Lipid Nanoparticles

Geisler, Ramsia; Pedersen, Martin Cramer; Hannappel, Yvonne; Schweins, Ralf; Prévost, Sylvain; Dattani, Rajeev; Arleth, Lise; Hellweg, Thomas

Mixtures of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the saponin ?-aescin spontaneously form monodisperse, bilayered discoidal micelles (also known as “bicelles” or “nanodisks”) in aqueous solution. Such bicelles form below the melting temperature of DMPC when the phospholipids are in the rigid L?? state and are precursors of spontaneously formed vesicles.…