Category

Lipids

ApplicationCharacteristic dimensionCharacterizationCustomer publicationsLipidsMaterialProteins & DNA/RNASAXSSizeStructural biologyTechnique

Ultrastructural modeling of small angle scattering from photosynthetic membranes

Jakubauskas, Dainius; Kowalewska, Lucja; Sokolova, Anna V.; Garvey, Christopher J.; Mortensen, Kell; Jensen, Poul Erik; Kirkensgaard, Jacob J. K.

The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation.…
12 March 2021
ApplicationCharacterizationCustomer publicationsFoodFood scienceGIWAXSLipidsMaterialPhase analysisSAXSSurface structure and patternsTechniqueWAXS

Physical Chemical Properties of Shea/Cocoa Butter Blends and their Potential for Chocolate Manufacture

Rodriguez-Negrette, Ana Carolina; Huck-Iriart, Cristián; Herrera, María Lidia

Cocoa butter (CB) is the preferred fat for chocolates and confections. However, for technological and economic reasons, there have been strong efforts for partially replacing it. As shea butter (SB) has become an important natural source of symmetrical stearic-rich triacylglycerols (TAG), the aim of this work was to study physical…
5 March 2021
ApplicationCharacterizationCustomer publicationsLipidsMaterialOtherPhase analysisSAXSSizeTechniqueTemperature

Antimicrobial and cytotoxic activity of (thio)alkyl hexopyranosides, nonionic glycolipid mimetics

Džubák, Petr; Gurská, So?a; Bogdanová, Kate?ina; Uhríková, Daniela; Kanjaková, Nina; Combet, Sophie; Klunda, Tomáš; Kolá?, Milan; Hajdúch, Marian; Poláková, Monika

A series of 19 synthetic alkyl and thioalkyl glycosides derived from d-mannose, d-glucose and d-galactose and having C10–C16 aglycone were investigated for cytotoxic activity against 7 human cancer and 2 non-tumor cell lines as well as for antimicrobial potential on 12 bacterial and yeast strains. The most potent compounds were…
27 January 2020
ApplicationCharacterizationCustomer publicationsLipidsMaterialPhase analysisSAXSSizeStructural biologyTechnique

Immobilisation of ?-galactosidase within a lipid sponge phase: structure, stability and kinetics characterisation

Gilbert, Jennifer; Valldeperas, Maria; Dhayal, Surender K.; Barauskas, Justas; Dicko, Cedric; Nylander, Tommy

In the formulation of an active enzyme enclosed in a matrix for controlled delivery, it is a challenge to achieve a high protein load and to ensure high activity of the protein. For the first time to our knowledge, we report the use of a highly swollen lipid sponge (L3)…
27 January 2020
(Hydro)gelsApplicationCharacteristic dimensionCharacterizationCustomer publicationsFood scienceLipidsMaterialSAXSSurfactantsTechnique

Development and characterization of a novel soy lecithin-stearic acid and whey protein concentrate bigel system for potential edible applications

Bollom, Mark A.; Clark, Stephanie; Acevedo, Nuria C.

Bigels are a new technology with great potential in the food industry. Their success with drug delivery suggests they may be able to deliver sensitive compounds in foods, such as probiotics and bioactives. The purpose of this study was to develop and characterize a novel, edible bigel system. The bigel…
27 January 2020
(Hydro)gelsApplicationCharacterizationCustomer publicationsLipidsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Effects of charge contrast and composition on microgel formation and interactions with bacteria-mimicking liposomes

Borro, Bruno C.; Toussaint, Marie S.; Bucciarelli, Saskia; Malmsten, Martin

Microgels offer opportunities for improved delivery of antimicrobial peptides (AMP). To contribute to a foundation for rational design of such systems, we here study the effects of electrostatics on the generation of peptide-carrying microgels. For this, alginate microgels loaded with polymyxin B and cross-linked by Ca2+, were formed by electrostatic…
27 January 2020
ApplicationCharacterizationCustomer publicationsLipidsMaterialParticle interactionsParticlesPhase analysisSAXSSizeStructural biologySurfactantsTechnique

Characterization of phospholipid vesicles containing lauric acid: physicochemical basis for process and product development

Farkuh, Laura; Hennies, Paulo T.; Nunes, Cláudia; Reis, Salette; Barreiros, Luisa; Segundo, Marcela A.; Oseliero Filho, Pedro L.; Oliveira, Cristiano L. P.; Cassago, Alexandre; Portugal, Rodrigo V.; Muramoto, Rodrigo A.; Carretero, Gustavo P. B.; Schreier, Shirley; Chaimovich, Hernan; Cuccovia, Iolanda M.

Lauric acid (LAH) strongly inhibits the growth of acne-causing bacteria. LAH is essentially water-insoluble and the solubility of laurate (LA) salts are medium and temperature dependent. Hence, LAH/LA preparations are difficult to formulate. Here we fully characterized phospholipid vesicles containing up to 50 mol% LAH. Vesicles of dipalmitoylphosphatidylcholine (DPPC) containing…
27 January 2020
ApplicationCharacteristic dimensionCharacterizationCustomer publicationsLipidsMaterialParticlesSAXSStructural biologyTechnique

Continuous, high-throughput production of artemisinin-loaded supramolecular cochleates using simple off-the-shelf flow focusing device

Shuddhodana; Wong, Pooi Wen Kathy; Judeh, Zaher

Lipid cochleates are gaining increasing interest as drug-carriers. However, their preparation relies on conventional batch processes that are complex, time consuming and lack batch-to-batch reproducibility; presenting a bottleneck for clinical translation. We report an efficient continuous preparation process for artemisinin-loaded cochleates (ART-cochleates) using inexpensive off-the-shelf flow…
27 January 2020
ApplicationCharacterizationCustomer publicationsLipidsMaterialPhase analysisSAXSShapeSizeStructural biologyTechnique

Effects of artepillin C on model membranes displaying liquid immiscibility

Pazin, W. M.; Vilanova, N.; Voets, I. K.; Soares, A. E. E.; Ito, A. S.

It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C,…
27 January 2020
ApplicationCharacterizationCustomer publicationsLipidsLiquid crystalsMaterialPhase analysisSAXSStructural biologyTechniqueTemperature

Cellular uptake of self-assembled phytantriol-based hexosomes is independent of major endocytic machineries

Rodrigues, Letícia; Schneider, Fabian; Zhang, Xiaohan; Larsson, Elin; Moodie, Lindon W. K.; Dietz, Hendrik; Papadakis, Christine M.; Winter, Gerhard; Lundmark, Richard; Hubert, Madlen

Despite increasing interests in non-lamellar liquid crystalline dispersions, such as hexosomes, for drug delivery, little is known about their interactions with cells and mechanism of cell entry. Here we examine the cellular uptake of hexosomes based on phytantriol and mannide monooleate by HeLa cells using live cell microscopy in comparison…