3D envelopes were determined from an automated sequence of measurements including 3 different proteins with low volume and dilute solutions.

Introduction

Small-angle X-ray scattering is complementary to other structural biology techniques. It provides reliable information on the shape of proteins in solution and associated parameters including macromolecule interactions and dynamics.
Reliability of the measurements and high throughput are key requirements to ensure the ability to run samples in a structural biology laboratory.
This note displays the quality of three-dimensional envelopes obtained on 3 protein samples (lysozyme, bovine serum albumin (BSA) and thyroglobulin) measured on the BioXolver through an automated measurement sequence.

Measurements & results

Concentration series, 3 concentrations per sample, were run successively using the in-line pipetting robot, which allows automatic sample loading and cell cleaning in a cycle time shorter than 2 minutes. 5 μL per sample condition were used for the measurements […]

High throughput protein envelope determination