(Hydro)gelsApplicationCharacterizationCustomer publicationsLipidsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Effects of charge contrast and composition on microgel formation and interactions with bacteria-mimicking liposomes

Borro, Bruno C.; Toussaint, Marie S.; Bucciarelli, Saskia; Malmsten, Martin

Microgels offer opportunities for improved delivery of antimicrobial peptides (AMP). To contribute to a foundation for rational design of such systems, we here study the effects of electrostatics on the generation of peptide-carrying microgels. For this, alginate microgels loaded with polymyxin B and cross-linked by Ca2+, were formed by electrostatic…
27 January 2020
ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeStructural biologyTechnique

Distinguishing Between Monomeric scFv and Diabody in Solution Using Light and Small Angle X-ray Scattering

Lüdel, Frank; Bufe, Sandra; Bleymüller, Willem M.; de Jonge, Hugo; Iamele, Luisa; Niemann, Hartmut H.; Hellweg, Thomas

Depending on the linker length between the V H and the V L domain, single-chain Fv (scFv) antibody fragments form monomers, dimers (diabodies) or higher oligomers. We aimed at generating a diabody of the anti-MET antibody 3H3 to use it as crystallization chaperone to promote crystallization of the MET ectodomain…
27 January 2020
ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologySurfactantsTechnique

Aescin-Induced Conversion of Gel-Phase Lipid Membranes into Bicelle-like Lipid Nanoparticles

Geisler, Ramsia; Pedersen, Martin Cramer; Hannappel, Yvonne; Schweins, Ralf; Prévost, Sylvain; Dattani, Rajeev; Arleth, Lise; Hellweg, Thomas

Mixtures of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the saponin ?-aescin spontaneously form monodisperse, bilayered discoidal micelles (also known as “bicelles” or “nanodisks”) in aqueous solution. Such bicelles form below the melting temperature of DMPC when the phospholipids are in the rigid L?? state and are precursors of spontaneously formed vesicles.…
27 January 2020
ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

Antigenic and physicochemical characterization of Hepatitis B surface protein under extreme temperature and pH conditions

Lopes, J. L. S.; Oliveira, D. C. A.; Utescher, C. L. A.; Quintilio, W.; Tenório, E. C. N.; Oliveira, C. L. P.; Fantini, M. C. A.; Rasmussen, M. K.; Bordallo, H. N.; Sant’Anna, O. A.; Botosso, V. F.

Hepatitis B virus causes acute and chronic infections in millions of people worldwide and, since 1982, a vaccine with 95% effectiveness has been available for immunization. The main component of the recombinant hepatitis B vaccine is the surface antigen protein (HBsAg). In this work, the effect of pH, ionic strength…
27 January 2020
ApplicationCharacterizationCustomer publicationsMaterialOtherProteins & DNA/RNASAXSShapeStructural biologyTechnique

Proinflammatory Action of a New Electronegative Low-Density Lipoprotein Epitope

Faulin, Tanize do Espirito Santo; Kazuma, Soraya Megumi; Tripodi, Gustavo Luis; Cavalcante, Marcela Frota; Wakasuqui, Felipe; Oliveira, Cristiano Luis Pinto; Degenhardt, Maximilia Frazão de Souza; Michaloski, Jussara; Giordano, Ricardo José; Ketelhuth, Daniel Francisco Jacon; Abdalla, Dulcineia Saes Parra

The electronegative low-density lipoprotein, LDL (?), is an endogenously modified LDL subfraction with cytotoxic and proinflammatory actions on endothelial cells, monocytes, and macrophages contributing to the progression of atherosclerosis. In this study, epitopes of LDL (?) were mapped using a phage display library of peptides and monoclonal antibodies reactive to…
27 January 2020
(Hydro)gels(Natural) polymersApplicationCharacterizationCustomer publicationsMaterialNanoparticles & colloidsSAXSSelf-assembled materialShapeSizeTechnique

Solvent-non-solvent rapid-injection for preparing nanostructured materials from micelles to hydrogels

Lang, Chao; LaNasa, Jacob A.; Utomo, Nyalaliska; Xu, Yifan; Nelson, Melissa J.; Song, Woochul; Hickner, Michael A.; Colby, Ralph H.; Kumar, Manish; Hickey, Robert J.

Due to their distinctive molecular architecture, ABA triblock copolymers will undergo specific self-assembly processes into various nanostructures upon introduction into a B-block selective solvent. Although much of the focus in ABA triblock copolymer self-assembly has been on equilibrium nanostructures, little attention has been paid to the guiding principles of nanostructure…
27 January 2020
ApplicationCharacterizationCustomer publicationsFoodFood scienceMaterialProteins & DNA/RNASAXSSelf-assembled materialShapeSizeStructural biologyTechnique

The influence of pH, protein concentration and calcium ratio on the formation and structure of nanotubes from partially hydrolyzed bovine ?-lactalbumin

Geng, XiaoLu; Kirkensgaard, Jacob Judas Kain; Arleth, Lise; Otte, Jeanette; Ipsen, Richard

Formation of nanotubes from partially hydrolysed ?-lactalbumin (?-La) was investigated at five pH values, two concentrations of ?-La and two calcium levels. Nanotubes were formed under almost all combinations of the investigated factors, and for the first time the formation of nanotubes at low pH (4.0) and low protein concentration…
27 January 2020
ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeStructural biologyTechnique

The role of disaccharides for protein–protein interactions – a SANS study

Olsson, Christoffer; Swenson, Jan

The disaccharide trehalose has shown outstanding anti-aggregation properties for proteins, which are highly important for the possibility to treat neurodegenerative diseases, such as Alzheimer’s and Huntington’s disease. However, the role and mechanism of trehalose for such stabilising effects are still largely unknown, partly because a direct structural picture of how…
27 January 2020
ApplicationCharacterizationCustomer publicationsLipidsMaterialPhase analysisSAXSShapeSizeStructural biologyTechnique

Effects of artepillin C on model membranes displaying liquid immiscibility

Pazin, W. M.; Vilanova, N.; Voets, I. K.; Soares, A. E. E.; Ito, A. S.

It has been hypothesized that the therapeutic effects of artepillin C, a natural compound derived from Brazilian green propolis, are likely related to its partition in the lipid bilayer component of biological membranes. To test this hypothesis, we investigated the effects of the major compound of green propolis, artepillin C,…
27 January 2020