Structural biology

(Hydro)gelsApplicationCharacterizationCustomer publicationsMaterialSAXSShapeSizeStructural biologySurfactantsTechnique

Deoxycholic acid and l-Phenylalanine enrich their hydrogel properties when combined in a zwitterionic derivative

Travaglini, Leana; di Gregorio, Maria Chiara; Severoni, Emilia; D’Annibale, Andrea; Sennato, Simona; Tardani, Franco; Giustini, Mauro; Gubitosi, Marta; Del Giudice, Alessandra; Galantini, Luciano

Hypothesis Sodium Deoxycholate (NaDC) and Phenylalanine (Phe) are important biological hydrogelators. NaDC hydrogels form by lowering the pH or by increasing the ionic strength. Phe gels form from saturated solution by thermal induction and slow kinetics. The resulting gels hold great potential in medicine and biology as drug carriers and…
25 November 2019
ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeSizeStructural biologyTechnique

The domain swapping of human cystatin C induced by synchrotron radiation

Taube, Michal; Pietralik, Zuzanna; Szymanska, Aneta; Szutkowski, Kosma; Clemens, Daniel; Grubb, Anders; Kozak, Maciej

Domain swapping is observed for many proteins with flexible conformations. This phenomenon is often associated with the development of conformational diseases. Importantly, domain swapping has been observed for human cystatin C (HCC), a protein capable of forming amyloid deposits in brain arteries. In this study, the ability of short exposure…
25 November 2019
(Natural) polymersApplicationCharacterizationCustomer publicationsMaterialSAXSShapeSizeStructural biologySurfactantsTechnique

Block copolymers as bile salt sequestrants: intriguing structures formed in a mixture of an oppositely charged amphiphilic block copolymer and bile salt

Schillén, Karin; Galantini, Luciano; Du, Guanqun; Del Giudice, Alessandra; Alfredsson, Viveka; Carnerup, Anna M.; Pavel, Nicolae V.; Masci, Giancarlo; Nyström, Bo

To study the formation and characterize the structure of mixed complexes of oppositely charged block copolymers and surfactants are of great significance for practical applications, e.g., in drug carrier formulations that are based on electrostatically assisted assembly. In this context, biocompatible block copolymers and biosurfactants (like bile salts) are particularly…
25 November 2019
ApplicationCharacteristic dimensionCharacterizationCustomer publicationsLipidsMaterialProteins & DNA/RNASAXSStructural biologyTechnique

Alginate-coating of artemisinin-loaded cochleates results in better control over gastro-intestinal release for effective oral delivery

Shuddhodana; Judeh, Zaher

Development of new drug delivery systems for effective delivery of poorly water-soluble drugs is important. We report the development of cochleate-based formulation for the oral administration of poorly water-soluble artemisinin (ART). Cochleates offer great potential as vehicles for the oral delivery of ART due to their high drug encapsulation and…
25 November 2019
ApplicationCharacterizationCustomer publicationsMaterialSAXSSizeStructural biologySurfactantsTechnique

Dynamics of proteins confined in non-ionic bicontinuous microemulsions: a FCS study

Wrede, Oliver; Großkopf, Sören; Seidel, Thorsten; Hellweg, Thomas

In our present work we present an approach which allows one to confine proteins in structurally nearly identical bicontinuous microemulsions with systematically decreasing water domain size. It is shown that sub-diffusive behaviour occurs already at water domain sizes below 13 nm. However, above 13 nm normal diffusion is seen. Moreover,…
25 November 2019
ApplicationCharacterizationCustomer publicationsMaterialPorous materialSAXSShapeSizeStructural biologyTechnique

3D visualisation of hepatitis B vaccine in the oral delivery vehicle SBA-15

Rasmussen, Martin K.; Kardjilov, Nikolay; Oliveira, Cristiano L. P.; Watts, Benjamin; Villanova, Julie; Botosso, Viviane Fongaro; Sant’Anna, Osvaldo A.; Fantini, Marcia C. A.; Bordallo, Heloisa N.

Developing a technology that enables oral vaccines to work efficiently remains a considerable effort since a number of difficulties must be addressed. The key objective being to ensure the safe passage through the harsh conditions within the gastrointestinal tract, promoting delivery that induces enhanced immune response. In the particular case…
18 July 2019
ApplicationCharacteristic dimensionCharacterizationCustomer publicationsLipidsMaterialSAXSStructural biologyTechnique

Reactive oxygen species (ROS)-responsive nanomedicine for RNAi-based cancer therapy

Li, Yang; Bai, Hongzhen; Wang, Hebin; Shen, Youqing; Tang, Guping; Ping, Yuan

Although much effort has been dedicated to the development of efficient siRNA delivery for cancer therapy, delivery nanomaterials that can particularly respond to reactive oxygen species (ROS), which are overproduced in the tissue and mitochondria of cancer cells, are still rare for the clinical translation of RNA interference (RNAi)-based therapy.…
18 July 2019
Customer publicationsParticle size and size distributionProteins & DNA/RNASAXSShapeStructural biology

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument

S. Bucciarelli, S. R. Midtgaard, M. Nors Pedersen, S. Skou, L. Arleth and B. Vestergaard

Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes...
8 April 2019