Application notes-Pharma

SAXS acquisition continuously without any beamstop provides High Dynamic Range data.

No parasitic scattering from beamstop edges or detector window allows noise-free scattering at low q.

The direct beam is recorded simultaneously with the sample scattering profile during acquisition for accurate transmitted intensity measurement, used to obtain a precise absolute intensity normalization.

In addition, the direct beam profile thus measured is integrated in the data analysis (XSACT) to improve the accuracy of the results.

As a standard solution, Xeuss 3.0 integrates low maintenance microfocus beam delivery systems to provide very high flux levels previously only possible with high power rotating anode sources.
Advanced experiments such as kinetic studies or shape analysis of diluted samples can now be performed in the lab.

In addition, Xenocs X-ray beam delivery systems coupled with motorized scatterless collimation achieve high resolution (low Δq beam), which is essential for studying large characteristic dimensions or for performing accurate mesophase analysis.

The high useful flux together with the high resolution capability are achieved through the combination of:

To ensure the best quality of X-ray scattering measurements, including on diluted or low contrast samples, Xeuss 3.0 combines high flux with low noise technology, which is the fruit of more than 10 years of development.

Key integrated features are:

For applications requiring very fast kinetics Xeuss 3.0 can also be delivered with a MetalJet source.

The Q-Xoom features full range computer-controlled detector travel to automatically adjust the sample-to-detector distance offering maximum flexibility of measurement for experiment optimization.

The virtual detector increases the measuring capabilities offering surfaces of collection larger than 200 mm² x 200 mm² at any sample-to-detector distance independently of detector size with automatic data reconstruction.

The large surface of detection is beneficial to optimize the azimuthal coverage in case of anisotropic samples or to benefit from high resolution measurement settings by characterizing smaller length scales (larger wave vectors) at longer sample to detector distance by a detector offset.

Q-Xoom – enjoy the flexibility of full-range computer-controlled detector travel

Measure probe lengths as large as 4.5 microns together with nanoscale information

Optimize your experiment to any type of sample (absorption, fluorescence, characteristic dimensions)

The Nano-inXider integrates 15 years of R&D in the field of advanced components and instrument design to achieve an optimized balance of high X-ray flux on sample together with low parasitic scattering generated by the instrument.

Clean Beam Technology embeds the following key components and features:

Automatic cosmic background removal reduces the impact of ambient parasitic scattering.

The BioXolver can be installed with different source options.

It can integrate a MetalJet source to obtain the highest X-ray beam intensity possible in a laboratory instrument.

It can also be installed with the Genix3D, a bright microfocus low maintenance source with intensity levels only previously accessible with high power rotating anode sources. Such performance is achieved by combining the Genix3D long lasting source with new patented FOX 3D multilayer optics having a very high solid angle.

The BioXolver comes with windowless hybrid photon counting (HPC) detectors which allows for beamstopless measurements.

By operating without beamstop the direct beam is recorded all along the acquisition, which leads to an accurate transmission measurement used to obtain a precise absolute intensity normalization.

Moreover, operating without beamstop reduces the lowest scattering angle achievable as it is no more limited by beamstop size but is sample dependent.

The BioXolver uses state of the art collimation slits that greatly reduce noise and provide a clean beam.

High quality detection is achieved with hybrid photon counting (HPC) detectors integrated inside vacuum with no interfering window in front of detector sensor (windowless detector). This ensures that weakly scattering samples can also be measured at low concentrations.

Overall measuring background level of the instrument is further improved by proprietary automatic cosmic background removal algorithms suppressing high energy cosmics hitting the detector.

The BioXolver includes powerful data reduction BioXTAS RAW widely used by the BioSAXS community.

It enables standard data operation and data analysis (Rg, molecular weight, etc.), together with advanced analysis using ATSAS plugins.

For high throughput analysis, the automated analysis function analyzes and compiles the results for you.

The BioXolver will work with any HPLC system, simply position the HPLC next to the instrument and hook up the column to the BioCUBE flow cell. The in-line UV/Vis ensures accurate determination of the sample concentration exactly at the SAXS exposure position. The included analysis software automatically reduces and provides an easy overview of your entire SEC-SAXS run and makes further analysis easy.

In situ UV measurement directly in the measurement cell provides concentration information of the sample before and after X-ray exposure or during a SEC-SAXS experiment.

This is especially important in this case as the sample is diluted in the transport path from the (HPLC) column exit to the x-ray exposure position.

BioXolver可以配备不同的光源。

可以结合MetalJet光源，在实验室仪器中获得尽可能高的X射线光束强度。

也可以安装Genix3D，一种低维护成本的高亮度微聚焦光源，其强度以前只有高功率阳极转靶才能达到。这种性能通过结合Genix3D耐用光源与具有超高立体角、新的专利FOX 3D多层膜聚焦镜实现。

BioXolver配备了无窗口膜混合光子（HPC）探测器，能够实现无beamstop测试。

在无beamstop情况下，整个测试过程中一直记录直通光，通过准确的透射测试来得到准确的绝对强度校正。

此外，由于不受beamstop尺寸限制，而只与样品有关，无beamstp测试可得到更小的最小散射角度。

BioXolver采用了最先进的准直狭缝，极大地降低了噪音，从而得到了纯净光束。

通过在真空中利用混合光子计数（HPC）探测器，且探测器传感器（无窗口膜探测器）前面无干扰，实现了高质量的检测。这可确保弱散射样品也可以在低浓度下测量。

采用专用的宇宙背景自动去除法，抑制高能宇宙射线，进一步降低了仪器整体的背景噪音。

BioXolver适用于任何HPLC系统，只需将HPLC置于仪器旁边，并将色谱柱连接到BioCUBE流动池。在线UV/Vis能够确保在SAXS曝光位置精确测量样品浓度。自带的分析软件可自动简化概述整个SEC-SAXS运行过程，这使得进一步分析更加容易。

由于原位UV测试直接在测试池进行，在X射线曝光之前和之后或在SEC-SAXS测试中都能得到样品的浓度信息。

因为样品在从(HPLC)柱出口到X射线曝光位置的传输过程中会被稀释，所以在这种情况下，这一点尤为重要。

无管操作确保样品消耗量低至5 μl。这在同类仪器中是独一无二的。

每个样品首先由无管移液机械臂自动注入到BioCUBE测量池中，然后定位到光束中进行测量，并实时对其位置进行连续控制。

样品在孔盘中储存期间和在BioCUBE测试期间都始终保持在选定的温度下。

您还可以选择具有集成UV/Vis功能的BioCUBE。

利用机械视觉技术，BioXolver能够随时对样品进行数字化监控。

样品装载后就会被准确地定位在X射线光束中，同时持续反馈结果给自动控制软件来保持光束中的样品量不变。

Nano-inXider通过在先进组件和仪器设计上长达15年的研发，达到了在样品上高X射线通量与仪器产生的低寄生散射的最佳平衡。

纯净光技术具有以下主要组件和特点：

自动去除宇宙背景可以减少环境寄生散射的影响。

Q-Xoom–享受全范围计算机控制探测器行程的灵活性

测量尺度可达4.5微米，并获取纳米尺度信息

对任何种类样品(吸收，荧光，特征尺寸)的实验进行优化

无beamstop连续采集高动态范围SAXS数据。

杜绝来自Beamstop边缘或探测器窗口膜的寄生散射，使低q处的散射信号不受干扰。。

在采集过程中同时记录直通光和样品散射信号，得到精确的透射强度，用于获取精确的绝对强度校正。

另外，采集的直通光信号将整合到数据分析软件（XSACT），提高解析结果的准确性。

作为一个标准的解决方案，Xeuss 3.0集成低维护成本的高性能微聚焦光源，亮度达到了之前高功率旋转阳极靶光源才能实现的水平。
现在实验室中就可进行诸如动力学研究或稀释样品的纳米粒子形状分析的高级实验。

另外，通过结合Xenocs公司的X射线光束和自动无散射准直系统实现高分辨率（光束Δq低），这对研究大特征尺寸的样品或进行准确中间相的分析都非常重要。

通过以下组合实现高光通量和高分辨率：

为了保证X射线散射的最佳测试质量，包括稀释的或对比度低的样品，Xeuss 3.0将10多年研发的高光通量和低噪音技术相结合。

主要集成功能包括：

配备MetalJet光源，用于非常快的动力学研究。

Q-Xoom采用电脑控制探测器全程移动，自动调节样品到探测器距离，提供最大的测试灵活性来优化实验。

虚拟探测器增强测试能力：在任意的样品到探测器距离下，结合图谱重构技术，任何规格的探测器的采集面积都将大于200 mm² x 200 mm²。

超大探测面积有利于优化各向异性样品信号的方位角覆盖，还有利于高分辨率测试，例如在距离样品较远处用偏置的探测器采集到更大的散射矢量，以表征较小的尺度。

高通量解决方案

* 也可配备低能光源（钒，铬）

小焦斑解决方案

* 也可配备低能光源（V, Cr）

2D-3D光束成型X射线聚焦镜

高通量聚焦镜

小光斑聚焦镜

准直聚焦镜

1D光束成型X射线聚焦镜

高通量聚焦镜

准直聚焦镜

Xenocs凭借其在多层涂层、表面成型和先进的测量技术方面的丰富经验，开发了一系列高性能的XRF分析仪聚焦镜。

凭借其独特的复型技术，Xenocs已经开发了新的光束分析仪，提高了探测下限，增大了可检测量。

XRF聚焦镜

BioXolver具有强大的数据处理软件BioXTAS RAW，且广泛应用于BioSAXS用户群体。

它可进行基本的数据操作和数据分析(Rg、分子量等)，以及使用ATSAS插件的高级分析。

对于高通量分析，自动分析功能可以帮您分析和汇总实验结果。

Nano-inXider数据采集软件具有以下用户友好的互动界面:

无需任何用户干预，自动工作流程可以进行以下所有数据处理

XSACT：X射线散射分析和计算工具

Tubeless handling of sample volume down to 5 μl. This is unique on the market.

Each sample is first automatically injected into the BioCUBE measurement cell with the tubeless pipetting robot, then positioned into the beam for measurement with dynamic real-time continuous control of its position.

The sample is maintained at the selected temperature both in the well plate during storage and in the BioCUBE during measurements.

Optionally, the BioCUBE may also be provided with integrated UV/Vis capabilities.

Using machine vision technology, the BioXolver is able to keep a digital eye on the sample at all times.

Once loaded, the sample is positioned with high precision in the X-ray beam while continuous feedback is provided to the automated control software to keep the volume in place.

The Nano-inXider data acquisition software features a user friendly interactive interface with:

Without any user interaction, the automatic workflow enables for all data treatments:

XSACT: X-ray Scattering Analysis and Calculation Tool