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Application notes

Application notes

Functional foods characterization at the nanoscale using SAXS

- Application highlight - Development of nanocarriers for functional food delivery is an active area of research. Both the development and the production process require characterization techniques that provide information about how nutrient loading and processing conditions influence the final structure and stability of nanocarriers and their dispersion in food.…
24 January 2020
Application notes

Vesicles

Probing the effect of oxidation on model lipid bilayers. The vital function of cells depends strongly on cell membranes which enclose cellular compartments and separate the cell interior from the extracellular domain. Oxidative stress for example damages the cell membrane and is at the origin of many pathological conditions such…
25 June 2019
Application notes

Microgels

Screening of stimuli-responsive colloidal particles. Microgels are colloidal particles consisting of cross-linked 3-dimensional polymer networks. These particles have the ability to shrink or swell substantially in response to external stimuli such as temperature, pH, ionic strength and light, allowing triggered release of encapsulated bioactive molecules. They additionally protect these loaded…
18 June 2019
Application notes

Thorough investigation of oligomers during protein fibrillation

The transient nature of aggregates during the fibrillation process of α-synuclein is highlighted with in-house SAXS experiments using the BioXolver. Protein aggregation into fibrillar structures is associated with a variety of neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. It is becoming increasingly evident that the toxic species causing the…
19 March 2019
Application notes

In-lab SEC-SAXS for structural investigation of complex samples

SAXS data and 3D envelopes of small and large protein species are obtained through in-line gel filtration. Small-angle X-ray scattering (SAXS) is commonly used to investigate the solution structure of biological macromolecules. Highly accurate results can be obtained with monodisperse solutions devoid of aggregates and impurities. Many biological processes, however,…
19 March 2019
Application notes

Protein-protein interactions – from in vivo functionality to pharmaceutics and food science

Salt concentration and pH impact were studied on lysozyme and BSA systems respectively by investigating various parameters (osmotic second virial coefficient, folding state and 3D shape). Protein-protein interactions are relevant for various biological processes, such as transcription and signal transduction, but also for protein crystallization, stability and shelf-life of pharmaceutical…
19 March 2019
Application notes

High throughput protein envelope determination

3D envelopes were determined from an automated sequence of measurements including 3 different proteins with low volume and dilute solutions. Small-angle X-ray scattering is complementary to other structural biology techniques. It provides reliable information on the shape of proteins in solution and associated parameters including macromolecule interactions and dynamics. Reliability of the…
14 March 2019
Application notes

Size distribution of Gold Nanoparticles

Shape and size of gold nanoparticles (NPs) in suspension have been determined by SAXS measurement. NPs are used even at very low concentration in many applications such as coatings, cosmetics or drug delivery. The performance of the final product depends not only on the chemical nature of the NPs but…
31 January 2019