Type of articles

Size distributions of industrial nanoparticles have been determined in various forms for quality assessment and process monitoring. Nanoparticles are being produced at an increasing rate and have become an essential building block for the development of new materials with improved properties. The performance of the final products depends not only…

Probing the effect of oxidation on model lipid bilayers. The vital function of cells depends strongly on cell membranes which enclose cellular compartments and separate the cell interior from the extracellular domain. Oxidative stress for example damages the cell membrane and is at the origin of many pathological conditions such…

Screening of stimuli-responsive colloidal particles. Microgels are colloidal particles consisting of cross-linked 3-dimensional polymer networks. These particles have the ability to shrink or swell substantially in response to external stimuli such as temperature, pH, ionic strength and light, allowing triggered release of encapsulated bioactive molecules. They additionally protect these loaded…

The transient nature of aggregates during the fibrillation process of α-synuclein is highlighted with in-house SAXS experiments using the BioXolver. Protein aggregation into fibrillar structures is associated with a variety of neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. It is becoming increasingly evident that the toxic species causing the…

SAXS data and 3D envelopes of small and large protein species are obtained through in-line gel filtration. Small-angle X-ray scattering (SAXS) is commonly used to investigate the solution structure of biological macromolecules. Highly accurate results can be obtained with monodisperse solutions devoid of aggregates and impurities. Many biological processes, however,…

Salt concentration and pH impact were studied on lysozyme and BSA systems respectively by investigating various parameters (osmotic second virial coefficient, folding state and 3D shape). Protein-protein interactions are relevant for various biological processes, such as transcription and signal transduction, but also for protein crystallization, stability and shelf-life of pharmaceutical…