Correlating SAXS and Cryo-EM for High-Throughput Liposome and LNP Analysis

Advanced biophysical characterization can accelerate the development of emerging drug modalities like liposomes and lipid nanoparticles (LNPs). Cryogenic electron microscopy (cryo-EM) is widely employed to assess liposome lamellarity and LNP morphology but the technique has low data throughput. Small Angle X-ray Scattering (SAXS) offers complementary size and shape information of nanoparticles in solution with higher throughput, especially when combined with robotics-enabled automated sampling. In this webinar, we show how we correlated cryo-EM imaging with SAXS measurements to enable SAXS as a rapid screening tool in pharmaceutical process and formulation development for liposomes and LNPs.