Customer publicationsParticle size and size distributionProteins & DNA/RNASAXSShapeStructural biology

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument

S. Bucciarelli, S. R. Midtgaard, M. Nors Pedersen, S. Skou, L. Arleth and B. Vestergaard

Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes...
8 April 2019
(Hydro)gelsCharacteristic dimensionCompositesCustomer publicationsNanoparticles & colloidsSAXSShape

Smart release of antimicrobial ZnO nanoplates from a pH-responsive keratin hydrogel

Villanueva, María E.; Cuestas, María L.; Pérez, Claudio J.; Campo Dall′ Orto, Viviana; Copello, Guillermo J.

A smart antibacterial biomaterial based on a keratin hydrogel with pH-dependent behavior and Zinc Oxide nanoplates as biocide agent has been developed. The pH of a chronic wound is basic due to bacterial metabolism. Originally shrank at acid pH, keratin hydrogels swell upon contact with a bacterial contaminated media leading…
12 March 2019
Customer publicationsProteins & DNA/RNASAXSShapeStructural biology

Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components

Razew, Michal; Warkocki, Zbigniew; Taube, Michal; Kolondra, Adam; Czarnocki-Cieciura, Mariusz; Nowak, Elzbieta; Labedzka-Dmoch, Karolina; Kawinska, Aleksandra; Piatkowski, Jakub; Golik, Pawel; Kozak, Maciej; Dziembowski, Andrzej; Nowotny, Marcin

The mitochondrial RNA degradosome (mtEXO) plays an essential role in the regulation of mitochondrial gene expression and is composed of the 3′-to-5′ exoribonuclease Dss1 and the helicase Suv3. Here the authors present the RNA bound mtEXO crystal structure and give insights into its…
12 March 2019
ApplicationCharacterizationCustomer publicationsInorganic materialInorganic materialsMaterialSAXSShapeTechnique

Strengthening mechanisms in Fe-Al based ferritic low-density steels

Pramanik, Sudipta; Koppoju, Suresh; Anupama, A. V.; Sahoo, Balaram; Suwas, Satyam

Low-density steels with different aluminium contents have been investigated with an aim to examine the occurrence of different strengthening mechanisms leading to its higher strength. A composition corresponding to 6.8wt% aluminium has been studied to understand the underlying strengthening mechanisms. Different factors contributing to the strengthening mechanisms have been separately…
12 March 2019
ApplicationCharacterizationCustomer publicationsInorganic materialsMaterialNanoparticles & colloidsParticlesPorous materialSAXSShapeTechnique

Monodisperse hollow silica spheres: An in-depth scattering analysis

Ruckdeschel, Pia; Dulle, Martin; Honold, Tobias; Förster, Stephan; Karg, Matthias; Retsch, Markus

Herein, we fabricate hollow silica nanoparticles with exceptionally narrow size distributions that inherently possess two distinct length scales—tens of nanometers with regards to the shell thickness, and hundreds of nanometers in regards to the total diameter. We characterize these structures using dynamic and static light scattering (DLS and SLS), small…
12 March 2019
ApplicationCharacterizationCustomer publicationsMaterialProteins & DNA/RNASAXSShapeStructural biologyTechnique

Phosphorylation-induced conformational dynamics in an intrinsically disordered protein and potential role in phenotypic heterogeneity

Kulkarni, Prakash; Jolly, Mohit Kumar; Jia, Dongya; Mooney, Steven M.; Bhargava, Ajay; Kagohara, Luciane T.; Chen, Yihong; Hao, Pengyu; He, Yanan; Veltri, Robert W.; Grishaev, Alexander; Weninger, Keith; Levine, Herbert; Orban, John

Intrinsically disordered proteins (IDPs) that lack a unique 3D structure and comprise a large fraction of the human proteome play important roles in numerous cellular functions. Prostate-Associated Gene 4 (PAGE4) is an IDP that acts as a potentiator of the Activator Protein-1 (AP-1) transcription factor. Homeodomain-Interacting Protein Kinase 1 (HIPK1)…
12 March 2019
ApplicationCharacterizationCustomer publicationsInorganic materialInorganic materialsMaterialNanoparticles & colloidsParticlesSAXSShapeTechnique

Conduction Band Fine Structure in Colloidal HgTe Quantum Dots

Hudson, Margaret H.; Chen, Menglu; Kamysbayev, Vladislav; Janke, Eric M.; Lan, Xinzheng; Allan, Guy; Delerue, Christophe; Lee, Byeongdu; Guyot-Sionnest, Philippe; Talapin, Dmitri V.

HgTe colloidal quantum dots (QDs) are of interest because quantum confinement of semimetallic bulk HgTe allows one to synthetically control the bandgap throughout the infrared. Here, we synthesize highly monodisperse HgTe QDs and tune their doping both chemically and electrochemically. The monodispersity of the QDs was evaluated using small-angle X-ray…
12 March 2019
ApplicationCharacterizationCustomer publicationsInorganic materialInorganic materialsMaterialMetalsNanoparticles & colloidsParticle size and size distributionParticlesSAXSShapeTechnique

Quantitative Analysis of Semiconductor Nanocrystal Ensemble Optical Extinction

Staller, Corey M.; Agrawal, Ankit; Gibbs, Stephen L.; Cabezas, Camila A. Saez; Johns, Robert W.; Milliron, Delia J.

The optical extinction coefficients of localized surface plasmon resonance (LSPR) in doped semiconductor nanocrystals (NCs) have intensities determined by the free charge carrier concentration and the mechanisms for damping the oscillation of those free carriers. We investigate the dependence of the extinction coefficient of tin-doped indium oxide (ITO) NCs on…
12 March 2019