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Customer publicationsParticle size and size distributionProteins & DNA/RNASAXSShapeStructural biology

Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument

S. Bucciarelli, S. R. Midtgaard, M. Nors Pedersen, S. Skou, L. Arleth and B. Vestergaard

Coupling of size-exclusion chromatography with biological solution small-angle X-ray scattering (SEC-SAXS) on dedicated synchrotron beamlines enables structural analysis of challenging samples such as labile proteins and low-affinity complexes...
8 April 2019
Application notes

Thorough investigation of oligomers during protein fibrillation

The transient nature of aggregates during the fibrillation process of α-synuclein is highlighted with in-house SAXS experiments using the BioXolver. Protein aggregation into fibrillar structures is associated with a variety of neurodegenerative disorders, such as Parkinson’s and Alzheimer’s diseases. It is becoming increasingly evident that the toxic species causing the…
19 March 2019
Application notes

In-lab SEC-SAXS for structural investigation of complex samples

SAXS data and 3D envelopes of small and large protein species are obtained through in-line gel filtration. Small-angle X-ray scattering (SAXS) is commonly used to investigate the solution structure of biological macromolecules. Highly accurate results can be obtained with monodisperse solutions devoid of aggregates and impurities. Many biological processes, however,…
19 March 2019
Application notes

Protein-protein interactions – from in vivo functionality to pharmaceutics and food science

Salt concentration and pH impact were studied on lysozyme and BSA systems respectively by investigating various parameters (osmotic second virial coefficient, folding state and 3D shape). Protein-protein interactions are relevant for various biological processes, such as transcription and signal transduction, but also for protein crystallization, stability and shelf-life of pharmaceutical…
19 March 2019
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Arkema and the Nano-inXider

As a designer of materials and innovative solutions, Arkema shapes materials and creates new uses that accelerate customer performance in lightweight and design materials, biosourced materials, new energies, water management, solutions for electronics, and the performance and home insulation. For the development of new polymers, Arkema used to characterize materials…
15 March 2019
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High throughput protein envelope determination

3D envelopes were determined from an automated sequence of measurements including 3 different proteins with low volume and dilute solutions. Small-angle X-ray scattering is complementary to other structural biology techniques. It provides reliable information on the shape of proteins in solution and associated parameters including macromolecule interactions and dynamics. Reliability of the…
14 March 2019